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71.
Hennigs Jan K. Lüneburg Nicole Stage Annett Schmitz Melanie Körbelin Jakob Harbaum Lars Matuszcak Christiane Mienert Julia Bokemeyer Carsten Böger Rainer H. Kiefmann Rainer Klose Hans 《Purinergic signalling》2019,15(3):299-311
Purinergic Signalling - Dysfunction of the pulmonary endothelium is associated with most lung diseases. Extracellular nucleotides modulate a plethora of endothelial functions in the lung such as... 相似文献
72.
Sandra Schlee Kristina Straub Thomas Schwab Thomas Kinateder Rainer Merkl Reinhard Sterner 《Proteins》2019,87(10):815-825
It is an important goal of computational biology to correctly predict the association state of a protein based on its amino acid sequence and the structures of known homologues. We have pursued this goal on the example of anthranilate phosphoribosyltransferase (AnPRT), an enzyme that is involved in the biosynthesis of the amino acid tryptophan. Firstly, known crystal structures of naturally occurring homodimeric AnPRTs were analyzed using the Protein Interfaces, Surfaces, and Assemblies (PISA) service of the European Bioinformatics Institute (EBI). This led to the identification of two hydrophobic “hot spot” amino acids in the protein-protein interface that were predicted to be essential for self-association. Next, in a comprehensive multiple sequence alignment (MSA), naturally occurring AnPRT variants with hydrophilic or charged amino acids in place of hydrophobic residues in the two hot spot positions were identified. Representative variants were characterized in terms of thermal stability, enzymatic activity, and quaternary structure. We found that AnPRT variants with charged residues in both hot spot positions exist exclusively as monomers in solution. Variants with hydrophilic amino acids in one hot spot position occur in both forms, monomer and dimer. The results of the present study provide a detailed characterization of the determinants of the AnPRT monomer-dimer equilibrium and show that analysis of hot spots in combination with MSAs can be a valuable tool in prediction of protein quaternary structures. 相似文献
73.
Sebastian Tesche Ren Rsemeier‐Scheumann Jonas Lohr Ren Hanke Jochen Büchs Rainer Krull 《Engineering in Life Science》2019,19(11):781-794
Salt‐enhanced cultivation as a morphology engineering tool for the filamentous actinomycete Actinomadura namibiensis was evaluated in 500‐mL shaking flasks (working volume 100 mL) with the aim of increasing the concentration of the pharmaceutically interesting peptide labyrinthopeptin A1. Among the inorganic salts added to a complex production medium, the addition of (NH4)2SO4 led to the highest amount of labyrinthopeptin A1 production. By using 50 mM (NH4)2SO4, the labyrinthopeptin A1 concentration increased up to sevenfold compared to the non‐supplemented control, resulting in 325 mg L?1 labyrinthopeptin A1 after 10 days of cultivation. The performance of other ammonium‐ and sulfate‐containing salts (e.g., NH4Cl, K2SO4) was much lower than the performance of (NH4)2SO4. A positive correlation between the uptake of glycerol as one of the main carbon sources and nongrowth‐associated labyrinthopeptin productivity was found. The change in the cell morphology of A. namibiensis in conjunction with increased osmolality by the addition of 50 mM (NH4)2SO4, was quantified by image analysis. A. namibiensis always developed a heterogeneous morphology with pellets and loose mycelia present simultaneously. In contrast to the non‐supplemented control, the morphology of (NH4)2SO4‐supplemented cultures was characterized by smaller and circular pellets that were more stable against disintegration in the stationary production phase. 相似文献
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Casartelli Alberto Melino Vanessa J. Baumann Ute Riboni Matteo Suchecki Radoslaw Jayasinghe Nirupama S. Mendis Himasha Watanabe Mutsumi Erban Alexander Zuther Ellen Hoefgen Rainer Roessner Ute Okamoto Mamoru Heuer Sigrid 《Plant molecular biology》2019,99(4-5):477-497
Plant Molecular Biology - Degradation of nitrogen-rich purines is tightly and oppositely regulated under drought and low nitrogen supply in bread wheat. Allantoin is a key target metabolite for... 相似文献
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77.
Zenderland Jasmine Hart Robbie Bussmann Rainer W. Paniagua Zambrana Narel Y. Sikharulidze Shalva Kikvidze Zaal Kikodze David Tchelidze David Khutsishvili Manana Batsatsashvili Ketevan 《Economic botany》2019,73(3):293-303
Economic Botany - The Use of “Use Value”: Quantifying Importance in Ethnobotany. Use value (UV) is an index widely used to quantify the relative importance of useful plants. It combines... 相似文献
78.
Medinets Sergiy Gasche Rainer Kiese Ralf Rennenberg Heinz Butterbach-Bahl Klaus 《Plant and Soil》2019,445(1-2):335-348
Plant and Soil - Soils are known to be significant sources of atmospheric nitric oxide (NO), a key compound in atmospheric chemistry. NO is a key regulating substance for inter- and intra-species... 相似文献
79.
Jacynthe Dessureault-Rompré Bernd Nowack Rainer Schulin Jörg Luster 《Plant and Soil》2006,286(1-2):99-107
Root–soil interactions can strongly influence the soil solution chemistry in the rhizosphere. In the present study we propose a modification of the classical rhizobox/micro suction cup system to make it suitable for the collection and analysis of organic acids in the rhizosphere. In order to show the potential of the method, we tested the modified system with Lupinus albus L. as a model plant known to exude large amounts of citrate. The suction cups were installed through the transparent front plate of the rhizoboxes just after the emergence of cluster roots in order to allow optimal localized collection of soil solution. A small dead-volume allowed almost immediate stabilisation with formaldehyde of the sampled soil solutions in the collection container to prevent microbial degradation. The concentrations of organic acids were significantly larger in the rhizosphere soil solution of active cluster roots of Lupinus albus L. than in the bulk soil solution (about 400 μM of citrate versus <0.05 μM). We were able to follow the exudation process in-situ, which occurred during 2–3 days. Also the concentrations of other organic acids and inorganic anions differed between the bulk soil and the rhizosphere of cluster roots, normal roots, and nodules. 相似文献
80.